<?xml version="1.0" encoding="utf-8"?><!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd"><article article-type="research-article" dtd-version="1.3" xml:lang="en" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:ali="http://www.niso.org/schemas/ali/1.0/"><front><journal-meta><journal-id journal-id-type="publisher-id">GBS</journal-id><journal-id journal-id-type="nlm-ta">Glob Biosecur</journal-id><journal-title-group><journal-title>Global Biosecurity</journal-title><abbrev-journal-title abbrev-type="pubmed">Glob Biosecur</abbrev-journal-title></journal-title-group><issn pub-type=”epub”> 2652-0036</issn><publisher><publisher-name>UNSWt</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">2024 6(1)</article-id><title-group><article-title>HIV Sero-Prevalence among Tuberculosis Patients in Bangladesh: A nationwide cross-sectional study</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Riaz</surname><given-names>Baizid Khoorshid</given-names></name><xref ref-type="aff" rid="aff1">¹</xref></contrib><contrib contrib-type="author"><name><surname>Amin</surname><given-names>Md. Kamrul</given-names></name><xref ref-type="aff" rid="aff1">¹</xref></contrib><contrib contrib-type="author"><name><surname>Islam</surname><given-names>Md. Shamiul</given-names></name><xref ref-type="aff" rid="aff2">²</xref></contrib><contrib contrib-type="author"><name><surname>Islam</surname><given-names>Shamsul</given-names></name><xref ref-type="aff" rid="aff1">¹</xref></contrib><contrib contrib-type="author"><name><surname>Akhter</surname><given-names>Jabin</given-names></name><xref ref-type="aff" rid="aff1">¹</xref></contrib><contrib contrib-type="author"><name><surname>Jahan</surname><given-names>Naznin Akter</given-names></name><xref ref-type="aff" rid="aff1">¹</xref></contrib><contrib contrib-type="author"><name><surname>Farhana</surname><given-names>Nasreen</given-names></name><xref ref-type="aff" rid="aff1">¹</xref></contrib><contrib contrib-type="author"><name><surname>Choudhury</surname><given-names>Rafaat</given-names></name><xref ref-type="aff" rid="aff1">¹</xref></contrib><contrib contrib-type="author"><name><surname>Uddin</surname><given-names>Mohammad Jamal</given-names></name><xref ref-type="aff" rid="aff1">¹</xref></contrib><contrib contrib-type="author"><name><surname>Alam</surname><given-names>Ummul Khair</given-names></name><xref ref-type="aff" rid="aff1">¹</xref></contrib><contrib contrib-type="author"><name><surname>Refat</surname><given-names>Md. Nazmul Hassan</given-names></name><xref ref-type="aff" rid="aff1">¹</xref></contrib><contrib contrib-type="author"><name><surname>Sobhan</surname><given-names>Sadia</given-names></name><xref ref-type="aff" rid="aff1">¹</xref></contrib><contrib contrib-type="author"><name><surname>Nahar</surname><given-names>Kamrun</given-names></name><xref ref-type="aff" rid="aff1">¹</xref></contrib><contrib contrib-type="author"><name><surname>Noor</surname><given-names>Irfan Nowroze</given-names></name><xref ref-type="aff" rid="aff1">¹</xref></contrib><contrib contrib-type="author"><name><surname>Ashad-Uz-Zaman</surname><given-names>Md. Noor</given-names></name><xref ref-type="aff" rid="aff1">¹</xref></contrib><contrib contrib-type="author"><name><surname>Nasreen</surname><given-names>Fatima</given-names></name><xref ref-type="aff" rid="aff1">¹</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Rahaman</surname><given-names>Mohammad Meshbahur</given-names></name><xref ref-type="aff" rid="aff1">¹</xref><xref ref-type="corresp" rid="cor1">^*</xref></contrib><contrib contrib-type="author"><name><surname>Khanam</surname><given-names>Fahmida</given-names></name><xref ref-type="aff" rid="aff1">¹</xref></contrib><aff id="aff1"><label>1</label><institution>National Institute of Preventive and Social Medicine (NIPSOM), Mohakhali</institution>, <addr-line><city>Dhaka</city>-<postal-code>1212</postal-code></addr-line>, <country country="bd">Bangladesh</country>.</aff><aff id="aff2"><label>2</label><institution>Mycobacterial Disease Control (MBDC), Tuberculosis-Leprosy and AIDS STD Programme (TB-L &amp; ASP) Unit of the Directorate General of Health Services (DGHS)</institution>, <country country="bd">Bangladesh</country></aff></contrib-group><author-notes><corresp id="cor1"><label>*</label><bold>Corresponding author</bold>: Mohammad Meshbahur Rahman, Assistant Professor, Department of Biostatistics, National Institute of Preventive and Social Medicine (NIPSOM), Mohakhali, Dhaka-1212, Bangladesh. Email: <email xlink:href="meshbah037@gmail.com">meshbah037@gmail.com</email>; Contact: +8801751509801</corresp></author-notes><pub-date date-type="pub" publication-format="electronic"><day>29</day><month>07</month><year>2024</year></pub-date><volume>6</volume><issue>1</issue></article-meta></front><body><sec sec-type="other1"><title>Abstract</title><sec sec-type="intro"><title>Introduction</title><p>Tuberculosis (TB) and human immunodeficiency virus (HIV) co-infection is a significant public health problem worldwide. In Bangladesh, the incidence rate of HIV-positive TB cases was 0.43 per 100,000 in 2020. Therefore, this study aimed to assess a nationwide sero-survey to determine the prevalence of HIV infection among TB patients to help the collaborative TB/HIV activities for achieving target 3.3 of the Sustainable Development Goals (SDGs) by 2030.</p></sec><sec sec-type="methods"><title>Methods</title><p>The cross-sectional survey utilized a two-stage probability proportional to size (PPS) systematic random sampling technique for selection of TB reporting centers (TRCs) from June 2020 to December 2021. The estimated 12,065 diagnosed tuberculosis patients, according to the NTP diagnostic algorithm, irrespective of age and sex, were included. The required information was collected through face-to-face interviews and record reviews using a pre-tested electronic TAB-based semi-structured questionnaire. With all aseptic precautions for all respondents who gave consent, 5 ml. venous blood was collected for the standard confirmatory test by 4th generation ELISA method for detection of antibody of HIV1/2/P24 antigen.</p></sec><sec sec-type="results"><title>Results</title><p>Out of 12,065 TB patients surveyed, most of them were from the Dhaka division (25%), urban (76%), 55 years and above (28%), male (56%), married (82%), illiterate (36%) and living in a nuclear family (70%). A total of 12 (0.1%) HIV-positive cases were found among TB patients across the country, and the majority (33%) of them belonged to the age group of 35 to 44 years, male (58%), lower educational group (67%), urban resident (67%), and from nuclear family (77%). The HIV-positive patients found more among pulmonary TB patients (83%) who were detected bacteriologically positive (58%) but smear and gene expert negative (75%). Among them, none was found to have progressed to drug-resistant TB.</p></sec><sec sec-type="conclusions"><title>Conclusion</title><p>HIV affects the immune system, and TB is one of the leading causes of death in HIV-infected people. Although the rate is low, there is a need for continued efforts to prevent and treat TB in Bangladesh, particularly among older age groups, urban residents, and those with co-morbidities such as diabetes.</p><p><bold>Keywords:</bold> Prevalence; Human immunodeficiency virus; Tuberculosis; Sero-survey; Bangladesh.</p></sec></sec><sec sec-type="intro"><title>Introduction</title><p>Tuberculosis (TB) is a serious public health concern, ranking among the top 13^th causes of death globally due to a single infectious agent [<xref ref-type="bibr" rid="r1">1</xref>]. In 2018, 10 million global people were diagnosed with TB, with men, women, and children being affected equally [<xref ref-type="bibr" rid="r2">2</xref>]. People living with HIV accounted for 9% of TB cases, and there were 160 new and recurrent cases reported per 100,000 people [<xref ref-type="bibr" rid="r1">1</xref>,<xref ref-type="bibr" rid="r2">2</xref>]. Despite progress in reducing TB deaths, an estimated 47,000 deaths occurred in 2018 [<xref ref-type="bibr" rid="r3">3</xref>]. In Bangladesh, a low-middle-income country with a population density of 1,115.62 people per square kilometre, 357,000 individuals were diagnosed with TB, with 90,000 cases remaining undiagnosed or unreported [<xref ref-type="bibr" rid="r4">4</xref>,<xref ref-type="bibr" rid="r5">5</xref>]. HIV is one of the top five risk factors for TB in Bangladesh, with 730 (360-1200) people living with TB/HIV, of which only 67 cases were reported in 2018 [<xref ref-type="bibr" rid="r1">1</xref>,<xref ref-type="bibr" rid="r5">5</xref>].</p><sec><title>Global scenario</title><p>Globally, tuberculosis and HIV co-infection is a significant health concern as it negatively impacts the outcome of both diseases [<xref ref-type="bibr" rid="r6">6</xref>,<xref ref-type="bibr" rid="r7">7</xref>]. TB is an opportunistic infection that is more prevalent in people with weakened immune systems, increasing the risk of TB in people with HIV. Infection with both HIV and TB is called HIV/TB co-infection, as suggested by numerous literatures [<xref ref-type="bibr" rid="r6">6</xref>-<xref ref-type="bibr" rid="r10">10</xref>]. TB-HIV co-infection can vary from 0-15% reported in a study conducted on the people of the European Union [<xref ref-type="bibr" rid="r6">6</xref>]. The risk factors for TB/HIV co-infection include sex, age, smoking, intravenous drug use, CD4 count at HIV diagnosis, and HIV transmission route [<xref ref-type="bibr" rid="r6">6</xref>,<xref ref-type="bibr" rid="r11">11</xref>,<xref ref-type="bibr" rid="r12">12</xref>]. However, improving early detection of TB/HIV co-infection can improve treatment outcomes [<xref ref-type="bibr" rid="r8">8</xref>,<xref ref-type="bibr" rid="r13">13</xref>]. In many countries, monitoring HIV levels in TB patients is important to address the spread of HIV and provide comprehensive care to those with TB/HIV.</p></sec><sec><title>Bangladesh perspectives</title><p>Bangladesh has achieved significant improvements in the public health sector through the reduction of child mortality, improvement of geriatric health, control of communicable and non-communicable diseases, and effective management of the deadly COVID-19 pandemic [<xref ref-type="bibr" rid="r14">14</xref>-<xref ref-type="bibr" rid="r20">20</xref>]. According to the SDGs set forth for Bangladesh, Goal 3, Target 3.3 aims to put an end to the outbreaks of AIDS, tuberculosis, malaria, and neglected tropical diseases by the year 2030 [<xref ref-type="bibr" rid="r21">21</xref>]. The diagnosis of HIV in TB patients is a challenge as a low-level epidemic, particularly in areas with limited access to healthcare and needs to prioritize the integration of TB/HIV services [<xref ref-type="bibr" rid="r22">22</xref>]. Previous studies indicate that the overall HIV prevalence in Bangladesh is less than 1% among most at-risk populations [<xref ref-type="bibr" rid="r23">23</xref>-<xref ref-type="bibr" rid="r27">27</xref>].</p><p>The absence of nationally representative information on the prevalence of HIV among tuberculosis patients is a critical issue in fulfilling the growing commitment to provide comprehensive HIV care, including antiretroviral therapy (ART), to individuals who are both HIV-positive and suffering from tuberculosis [<xref ref-type="bibr" rid="r25">25</xref>]. Testing for HIV in TB patients and their contacts often results in a high number of new diagnoses. However, often, the treatment is not offered or taken up. In Bangladesh, TB care and treatment services are integrated into the primary healthcare system, but only 17% of the estimated number of TB patients living with HIV receive ART [<xref ref-type="bibr" rid="r28">28</xref>]. The National AIDS/STD Program (NASP), within the Directorate General of Health Services (DGHS) of the Ministry of Health and Family Welfare (MoHFW), acts as a nodal body responsible for programming to address HIV issues in the country [<xref ref-type="bibr" rid="r26">26</xref>]. According to WHO regional health statistics, the number of new HIV infections was 0.01 per 1,000 uninfected population and has been stable since 1990 [<xref ref-type="bibr" rid="r29">29</xref>]. The National TB Control Program in Bangladesh works closely with other organizations to provide high-quality, integrated TB care and prevention [<xref ref-type="bibr" rid="r26">26</xref>].</p><p>The country is among the world&apos;s 30^th highest TB burden countries and needs information about its HIV epidemic to combat its spread [<xref ref-type="bibr" rid="r30">30</xref>]. This study conducted a nationally representative sero-survey to determine the prevalence of HIV infection among tuberculosis patients in Bangladesh. The study is crucial for Bangladesh to fulfil the target of the Sustainable Development Goals and increase service coverage to achieve Universal Health Coverage.</p></sec></sec><sec sec-type="methods"><title>Methods</title><sec><title>Ethical Clearance and permission of Data Collection</title><p>Ethical approval for the study was obtained from the Institutional Review Board of Bangladesh Medical Research Council (BMRC), with registration number BMRC/NREC/2019-2022/993. A brief introduction to the aims and objectives of the study was given first. Then, the written consent translated into the native language was read to illiterate tribal elderly. All collected data was kept anonymously. The design and delivery of data collection for this study continuously tried to ensure the enrolled population&apos;s rights to privacy, confidentiality, informed consent and freedom of movement. The information was kept with the highest confidentiality and was used only for this study, and privacy was maintained during data collection. The participants were given full freedom to withdraw their consent to participate at any stage of the study. The national TB program was also proactively engaged, empowering patients in study design, data collection, and service delivery to accountability.</p></sec><sec><title>Study setting, population, and pre-testing</title><p>This cross-sectional survey study was conducted from June 2020 to December 2021. In this design, data was collected through interviews, a questionnaire, document review, and blood sample collection. According to 1^st and 2^nd quarter 2020 data, a total of 100163 patients are getting treatment registered under 857 countrywide TRCs. So, the target population for this survey includes all patients suffering from tuberculosis and registered under the TB reporting centre of the National Tuberculosis Control Programme (NTP) from 1^st quarter to 3^rd quarter of 2020, including new diagnoses. Pre-testing was conducted by six (06) teams at the end of September 2020. A total of 81 respondents&apos; data and biological samples were collected. Response rates were 35% in urban centres while only 100% in rural centres during pre-testing. All the samples were found to be negative for HIV.</p></sec><sec><title>Data Collection Technique:</title><p>After getting verbal and written consent, data was collected by trained laboratory technicians from the participants by face-to-face interviews through TAB based pre-tested, semi-structured questionnaire. For the HIV test, blood was collected from the participants by following standard guidelines, and the sample was tested at the laboratory of the National Institute of Preventive and Social Medicine.</p></sec><sec><title>Team composition</title><p>The technical committee of the survey consisted of one principal investigator, two co-principal investigators and thirteen co-investigators. Besides these, one finance and budget officer, one administrative officer, one research officer, two quality control officers, one statistician, ten field supervisors (Public Health Background) and thirty data enumerators (MT lab technician/phlebotomist) were recruited for the survey.</p></sec><sec><title>Training of Field Supervisors and Data Enumerators</title><p>Training of field supervisors and data enumerators was conducted in September 2020. All the training sessions were conducted by esteemed investigators, project officials, and other resource persons. The training ensured a common understanding of the terms and definitions used in the survey manual and ensured accurate data collection, tab management and good data quality. On the closing day, a feedback session about the questionnaire was arranged where the Line Director of NTP and Director of NIPSOM (Principal Investigator) graced the session.</p></sec><sec><title>Variables included in the study</title><p>Variables in this study were the socio-demographic variables [Age, Sex, Religion, Marital status, Education, Occupation, Family type], Co-morbidities [Diabetes, Hypertension, Kidney diseases, COPD], History of transfusion/infusion, History of any major or minor surgery, Past history, History of previous TB treatment, H/O contact with TB or HIV patients, Diagnosis-related variables, Process of TB-HIV co-infection diagnosis, Magnitude of TB-HIV co-infection in the catchment area, Notification process of TB-HIV co-infection, TB-HIV integrated service-related variables, and vulnerable group for TB-HIV co-infection according to focal persons. Respondents&apos; HIV sero-prevalence was observed by collecting blood samples.</p></sec><sec><title>Selection Criteria</title><p>Inclusion Criteria were diagnosed tuberculosis patients according to the NTP diagnostic algorithm irrespective of age and sex. Participants who provided informed written or verbal consent were included in the study. The target population of the study included individuals residing in all geographical areas of the country. As this survey was under NTP, the exclusion criteria were tuberculosis patients not enrolled under NTP and patients declared as cured/treatment completed.</p></sec><sec><title>Sample size estimation</title><p>From general theory, the minimum required sample size is determined using the usual sample size determination formula for estimating proportion with a relative margin of error, which is given by <inline-formula><mml:math id="m1"><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mfrac><mml:mrow><mml:msub><mml:mrow><mml:mi>n</mml:mi></mml:mrow><mml:mrow><mml:mn>0</mml:mn></mml:mrow></mml:msub></mml:mrow><mml:mrow><mml:mn>1</mml:mn><mml:mo>+</mml:mo><mml:mfrac><mml:mrow><mml:msub><mml:mrow><mml:mi>n</mml:mi></mml:mrow><mml:mrow><mml:mn>0</mml:mn></mml:mrow></mml:msub></mml:mrow><mml:mrow><mml:mi>N</mml:mi></mml:mrow></mml:mfrac></mml:mrow></mml:mfrac><mml:mo>&#x00D7;</mml:mo><mml:mi>d</mml:mi><mml:mi>e</mml:mi><mml:mi>f</mml:mi><mml:mi>f</mml:mi></mml:math></inline-formula>, (1)</p><disp-formula id="e2">with <mml:math id="m2"><mml:msub><mml:mrow><mml:mi>n</mml:mi></mml:mrow><mml:mrow><mml:mn>0</mml:mn></mml:mrow></mml:msub><mml:mo>=</mml:mo><mml:mfrac><mml:mrow><mml:msup><mml:mrow><mml:mi>z</mml:mi></mml:mrow><mml:mrow><mml:mn>2</mml:mn></mml:mrow></mml:msup><mml:mi>p</mml:mi><mml:mo>(</mml:mo><mml:mn>1</mml:mn><mml:mo>-</mml:mo><mml:mi>p</mml:mi><mml:mo>)</mml:mo></mml:mrow><mml:mrow><mml:msup><mml:mrow><mml:mi>d</mml:mi></mml:mrow><mml:mrow><mml:mn>2</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:mfrac></mml:math><label>(2)</label></disp-formula><p>Where &quot;p&quot; represents the prior estimated proportion of the desired characteristics in the population, &quot;z&quot; represents the value of a standard normal variate that provides a 100&#x03B1;% chance of obtaining a poor sample, &quot;d&quot; represents the acceptable relative margin of error, &quot;N&quot; is the total population size, and &quot;deff&quot; represents the design effect used in complex surveys that employ multi-stage cluster sampling.</p><p>Here, we have considered p = 0.001 (according to a survey of NTP, the estimated proportion of HIV among TB patients is 0.1% [<xref ref-type="bibr" rid="r25">25</xref>]), q = (1-p) = 0.999, z = 1.96 and d = 0.0006 (at 0.06%). From the 2019 Annual TB report, N= Total number of TB patients in a year= 100163. Considering a 10% unavailability in the course of the study time, we have N=0.9X100163= 90488. We consider design effect=1.25.</p><p>As a result, the required sample size becomes</p><disp-formula id="e"><mml:math id="m3"><mml:msub><mml:mrow><mml:mi>n</mml:mi></mml:mrow><mml:mrow><mml:mn>0</mml:mn></mml:mrow></mml:msub><mml:mo>=</mml:mo><mml:mfrac><mml:mrow><mml:msup><mml:mrow><mml:mi>z</mml:mi></mml:mrow><mml:mrow><mml:mn>2</mml:mn></mml:mrow></mml:msup><mml:mi>p</mml:mi><mml:mo>(</mml:mo><mml:mn>1</mml:mn><mml:mo>-</mml:mo><mml:mi>p</mml:mi><mml:mo>)</mml:mo></mml:mrow><mml:mrow><mml:msup><mml:mrow><mml:mi>d</mml:mi></mml:mrow><mml:mrow><mml:mn>2</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:mfrac><mml:mo>=</mml:mo><mml:mfrac><mml:mrow><mml:msup><mml:mrow><mml:mn>1.96</mml:mn></mml:mrow><mml:mrow><mml:mn>2</mml:mn></mml:mrow></mml:msup><mml:mo>&#x00D7;</mml:mo><mml:mn>0.0001</mml:mn><mml:mo>&#x00D7;</mml:mo><mml:mo>(</mml:mo><mml:mn>1</mml:mn><mml:mo>-</mml:mo><mml:mo>.</mml:mo><mml:mn>0001</mml:mn><mml:mo>)</mml:mo></mml:mrow><mml:mrow><mml:msup><mml:mrow><mml:mn>0.0006</mml:mn></mml:mrow><mml:mrow><mml:mn>2</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:mfrac><mml:mo>=</mml:mo><mml:mn>10660</mml:mn><mml:mo>.</mml:mo></mml:math> </disp-formula><disp-formula id="e___1">Hence <mml:math id="m4"><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mfrac><mml:mrow><mml:msub><mml:mrow><mml:mi>n</mml:mi></mml:mrow><mml:mrow><mml:mn>0</mml:mn></mml:mrow></mml:msub></mml:mrow><mml:mrow><mml:mn>1</mml:mn><mml:mo>+</mml:mo><mml:mfrac><mml:mrow><mml:msub><mml:mrow><mml:mi>n</mml:mi></mml:mrow><mml:mrow><mml:mn>0</mml:mn></mml:mrow></mml:msub></mml:mrow><mml:mrow><mml:mi>N</mml:mi></mml:mrow></mml:mfrac></mml:mrow></mml:mfrac><mml:mo>&#x00D7;</mml:mo><mml:mi>d</mml:mi><mml:mi>e</mml:mi><mml:mi>f</mml:mi><mml:mi>f</mml:mi><mml:mo>=</mml:mo><mml:mfrac><mml:mrow><mml:mn>10660</mml:mn></mml:mrow><mml:mrow><mml:mn>1</mml:mn><mml:mo>+</mml:mo><mml:mfrac><mml:mrow><mml:mn>10660</mml:mn></mml:mrow><mml:mrow><mml:mn>90488</mml:mn></mml:mrow></mml:mfrac></mml:mrow></mml:mfrac><mml:mo>&#x00D7;</mml:mo><mml:mn>1.25</mml:mn><mml:mo>=</mml:mo><mml:mn>11920</mml:mn><mml:mo>=</mml:mo><mml:mn>12000</mml:mn><mml:mo>(</mml:mo><mml:mi>a</mml:mi><mml:mi>p</mml:mi><mml:mi>p</mml:mi><mml:mi>x</mml:mi><mml:mo>)</mml:mo><mml:mo>.</mml:mo></mml:math> </disp-formula><p>The data from the first and second quarter of 2020 revealed that 100,163 patients received treatment at 857 Treatment and Recovery Centers (TRCs) across the country. After evaluating the current situation and patient recovery rate from the first quarter, it was determined that the number of patients would decrease by 10%. Based on this estimation, the expected number of patients at the field was 90,146, and the sample size was calculated to be 11,920. The final sample size was estimated to be approximately 12,000.</p></sec><sec><title>Sampling techniques</title><p>The survey was performed by probability-to-proportion sampling in two stages.</p><p><bold>Stage I:</bold> Selection of TRC from 64 districts was done by probability proportion sampling (PPS) based on the list of TRCs all over the country as primary sampling units. For the first stage of PPS systematic sampling, we first determined the number of centres to be selected, assuming an average of 50 patients from each centre. In the second stage, we need 238 centres. Since in PPS systematic sampling, some centres may have missed out on the selection; we started with a 10% higher number of centres, which is 263. Attempting 263 centres, the PPS systematic sampling ended up selecting 253 centres.</p><p><bold>Stage II</bold>: The selection of the sample patients was made through systematic random sampling from the selected TRCs, maintaining sample sizes proportional to the number of patients listed under the centre. At the same time, the second stage sampling frame was the patients&apos; registration records. Patients were selected systematically from each TRC with an interval of 4. Neither replacement nor changes of the pre-selected TRC or patient were allowed at the implementing stage to prevent bias.</p></sec><sec><title>Pre-testing</title><p>Pre-testing was conducted by six (06) teams in three rural TRCs (Savar Upzilla Health Complex (UHC), Dhamrai UHC, Dhaka Export Processing Zone DOTs centre) and three from urban (Malibag BGME TRC, Lalbag TRC and Naare Maitri TRC, Mohakhali, Dhaka). Each team consists of three data enumerators led by one supervisor and two co-investigators. A total of 81 respondents were observed in pre-testing, with a response rate of 65% in urban centres and 100% in rural centres. All the samples were found to be negative for HIV. The questionnaire was then corrected and modified according to the findings after pre-testing.</p></sec><sec><title>Data collection</title><p>A trained data enumerators team was employed in data collection. Data was collected through face-to-face interviews using a pre-tested electronic TAB-based semi-structured questionnaire. A document review was done to collect treatment and investigation-related data. To observe the HIV prevalence, blood samples were collected from 12065 participants after getting their consent and were tested at NIPSOM LAB. Maintaining cold chain and taking all aseptic precautions for all respondents who gave consent, 5 ml. venous blood was collected to test HIV, upholding the standard confirmatory test by 4th generation ELISA method for detection of antibody of HIV1/2/P24 antigen. External quality assurance was done on all positive samples and 1% of negative samples in the HIV reference laboratory. Proper aseptic precautions were taken, and all the samples were coded properly during blood sample collection. The whole data collection process was supervised by the trained supervisor, and the technical committee visited the data collection field frequently to ensure valid data collection. The collected data was processed in the central office, NIPSOM, for authenticity, consistency and relevancy.</p><p>To prevent any unwanted harm or complication during the sample collection and waste management, all the phlebotomists were trained extensively to ensure their safety first. The necessary biosafety procedure for personal protective equipment was maintained properly. All kinds of logistics were provided to maintain standard procedures for sample collection. In case of any accidental hazard or damage during their work in the field, the principal investor was responsible for ensuring the proper treatment and management for both respondents and phlebotomists. In addition, as the respondents gave us their valuable 15 minutes during data collection, as compensation, a refreshment was arranged for all the respondents coming to TRCs with the help of the local authority.</p></sec></sec><sec sec-type="other2"><title>Data analysis</title><p>In data analysis, we performed a frequency distribution table, different graphs, and sub-group descriptive analysis. The socio-demographic profile and co-morbidities of the respondents were analyzed through a frequency distribution table. The prevalence of HIV among tuberculosis patients was presented in a simple bar diagram. A sub-group analysis for the HIV-positive cases was also done by descriptive statistics. All the statistical analysis was performed by SPSS-25 version.</p></sec><sec sec-type="results"><title>Results</title><sec><title>Socio-demographic profile of the respondents</title><p>Among the 12,065 tuberculosis patients included in the study, the majority, 28.4% (n=3421), were 55 years and older with a mean age of 41.90 years (standard deviation=16.96), followed by &lt;15 years 3.9% (n=469). The samples were collected from all eight administrative divisions of Bangladesh, with a maximum 25% (n=3020) TB patients from the Dhaka division, followed by 18% (n=2169) from Chattogram, 17.1% (n=2069) from Khulna, 10.0% (n=1206) from Rangpur, 9.6% (n=1159) from Sylhet, 7.1% (n=856) from Rajshahi, 7.0% (n=849) from Barisal and Mymensingh (6.1%) respectively. The majority, 75.85%, of the patients were urban residents, with 13.01% from Peri-urban and 11.14% from rural residences. More than half (56.0%) of the TB patients were male, and 43.8% were female, with a small portion (0.2%) being transgender. The majority, 82% (n=9898) of the patients were currently married, followed by 14.0% (n=1687) were unmarried, 3.4% (n=415) were widows or widowers, and 0.5% (n=65) were either separated or divorced. About 4327 (35.9%) of the TB patients were found to be illiterate, where 3081 (25.5%) had completed their primary education, 19.8% belonged to primary education, 1.5% had completed college or university, and 1.8% had completed post-graduation. The majority of the respondents were housewives (31.7%), followed by a farmer (18.4%), students (8.6%), Small business (8.3%), unemployed but able to work (6.8%) and day labourers (5.7%) respectively. The majority of the patients came from the nuclear family (70.9%), with 28.6% from joint family and a small portion (0.5%) were found out of the family (residing in hostels or mess etc.) (<xref ref-type="table" rid="t1">Table 1</xref>).</p><table-wrap id="t1" position="float"><label>Table 1</label><caption><title>Socio-demographic characteristics of the respondents.</title></caption><table frame="hsides" rules="groups"><col width="51.9%"/><col width="22.15%"/><col width="25.95%"/><thead><tr><th valign="top" align="left" scope="col" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt"><bold>Baseline characteristics</bold></th><th valign="top" align="left" scope="col" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt"><bold>Frequency (n)</bold></th><th valign="top" align="left" scope="col" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt"><bold>Percentage (%)</bold></th></tr></thead><tbody><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Age group (mean age=41.90; SD=16.96; Age range: 1 to 106 years)</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">&lt;15 years</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">469</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">3.9</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">15-24 years</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">1691</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">14</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">25-34 years</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">2101</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">17.4</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">35-44 years</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">2174</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">18</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">45-54 years</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">2209</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">18.3</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">55+ years</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">3421</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">28.4</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Division</bold></td></tr><tr><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Barisal</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">849</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">7.04</td></tr><tr><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Chattogram</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">2169</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">17.98</td></tr><tr><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Dhaka</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">3020</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">25.03</td></tr><tr><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Khulna</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">2069</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">17.15</td></tr><tr><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Mymensingh</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">737</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">6.11</td></tr><tr><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Rajshahi</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">856</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">7.09</td></tr><tr><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Rangpur</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">1206</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">10.00</td></tr><tr><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Sylhet</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">1159</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">9.61</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Residence</bold></td></tr><tr><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Rural</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">1344</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">11.14</td></tr><tr><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Peri urban</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">1570</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">13.01</td></tr><tr><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Urban</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">9151</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">75.85</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Sex</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Male</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">6762</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">56</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Female</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">5284</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">43.8</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Third gender</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">19</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">0.2</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Marital status</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Unmarried</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">1687</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">13.98</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Currently Married</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">9898</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">82.04</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Separate/Divorce</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">65</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">0.54</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Widow</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">415</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">3.44</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Education</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Illiterate</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">4327</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">35.86</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Less than Primary</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">2392</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">19.83</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Primary completed</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">3081</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">25.54</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Secondary Completed</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">1214</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">10.06</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Higher Secondary Completed</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">655</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">5.43</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">College/University Completed</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">181</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">1.50</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Post-Graduate Completed</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">214</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">1.77</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Not agree to discuss</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">1</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">0.01</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Occupation (categories having more than 5% of proportion)</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Housewife</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">3827</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">31.7</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Farmer</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">2214</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">18.4</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Students</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">1042</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">8.6</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Small Business</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">998</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">8.3</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Unemployed but Able to work</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">818</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">6.8</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Day Labors</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">690</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">5.7</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Type of family</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Nuclear Family</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">8551</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">70.9</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Joint</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">3449</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">28.6</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Out of Family</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">65</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">0.5</td></tr></tbody></table></table-wrap></sec><sec><title>Patients&apos; type and co-morbidities</title><p>Out of 12,065 respondents, 11778 (97.6%) of them were suffering from Tuberculosis (TB) and the rest 287 (2.4%) were found to be drag resistance tuberculosis (DRTB) patients. About 6788 (57.63%) of the patients had detected TB by Bacteriologically and the rest were clinically detected. More than eight out of ten patients were suffering from at least one co-morbidity where Diabetes (n=1119, 9.27%) was the more frequent followed by Hypertension (n=430, 3.56%), Asthma (n=386, 3.20%), COPD (n=244, 2.02%), Cancer (n=8, 0.07%), heart disease (n=201, 1.67%), CKD (n=48, 0.40%) and Thalassemia (n=7, 0.06%). COVID-19 was tested by 545 patients, and 4.22% (n=23) were prevalent (<xref ref-type="table" rid="t2">Table 2</xref>).</p><table-wrap id="t2" position="float"><label>Table 2</label><caption><title>Patient&apos;s type and existence of co-morbidity.</title></caption><table frame="hsides" rules="groups"><col width="56.87%"/><col width="24.43%"/><col width="18.7%"/><thead><tr><th valign="top" align="left" scope="col" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt"><bold>Characteristics</bold></th><th valign="top" align="left" scope="col" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt"><bold>Frequency</bold></th><th valign="top" align="left" scope="col" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt"><bold>Percent</bold></th></tr></thead><tbody><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Type of patients</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">TB</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">11778.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">97.62</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">DR-TB</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">287.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">2.38</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Type of patients&apos; detection (n=11778)</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Clinically</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">4990.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">42.37</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Bacteriologically</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">6788.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">57.63</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Presence of co-morbidity</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">One co-morbidity</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">1734.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">83.37</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Two co-morbidities</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">281.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">13.51</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Three or more co-morbidities</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">65.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">3.13</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Diabetes</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">No</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">10946.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">90.73</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Yes</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">1119.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">9.27</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Hypertension</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">No</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">11635.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">96.44</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Yes</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">430.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">3.56</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Chronic Obstructive Pulmonary Disease (COPD)</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">No</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">11821.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">97.98</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Yes</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">244.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">2.02</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Cancer</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">No</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">12057.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">99.93</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Yes</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">8.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">0.07</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Asthma</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">No</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">11679.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">96.80</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Yes</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">386.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">3.20</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Heart Disease</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">No</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">11864.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">98.33</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Yes</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">201.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">1.67</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Chronic kidney disease (CKD)</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">No</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">12017.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">99.60</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Yes</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">48.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">0.40</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Hepatitis</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">No</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">12008.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">99.53</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Yes</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">57.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">0.47</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Thalassemia</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">No</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">12058.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">99.94</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Yes</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">7.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">0.06</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Others co-morbidity</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">No</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">11865.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">98.34</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Yes</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">200.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">1.66</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>COVID-19 (n=545)</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Positive</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">23.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">4.22</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Negative</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">522.00</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt">95.78</td></tr></tbody></table></table-wrap></sec><sec><title>Prevalence of HIV among Tuberculosis patients</title><p>A total of 12 HIV-positive cases were found among 12065 tuberculosis patients across the country. The positive cases were verified by diagnosing from another laboratory. Therefore, the HIV sero-prevalence among tuberculosis in Bangladesh is 0.1% (<xref ref-type="fig" rid="f1">Figure 1</xref>).</p><fig id="f1" position="float" fig-type="figure"><label>Figure 1</label><caption><title>Prevalence of HIV among Tuberculosis patients</title></caption><graphic xlink:href="20246(1)-f1"></graphic></fig></sec><sec><title>Socio-demographic profile of HIV-positive patients</title><p>Out of 12 HIV-positive cases, majority 33.3% (n=4) patients were from the age group 35-44 years, followed by 25.0%(n=3) from 45-54 years, 16.7% (n=2) from 25-34 years and above 55 years, and 8.3%(n=1) from the age group 15- 24 years. Most of the HIV-positive cases were male (n=7, 58.3%), and 5 (41.7%) were female patients. HIV prevalence was found to be higher among the lower educational group. The illiterate and less than primary group contributed 33% each (4 from each group respectively). Three HIV-positive patients had completed up-to primary education, and one patient had completed secondary education. Eight (66.7%) out of 12 patients were currently married, followed by two (16.7%) were unmarried, one patient (8.3%) was separated or divorced, and one (8.3%) was a widow. The HIV-positive patients&apos; occupations were private service (n=1, 8.3%), small business (n=1, 8.3%), farmer (n=1, 8.3%), day labourers (n=2, 16.7%), transport labour (n=2, 16.7%), housewife (n=4, 33.3%) and others (n=1, 8.3%). Nine (75%) HIV-positive patients were from the nuclear family, and three (25%) were from joint families, where the majority (n=8, 66.6%) were from urban residents (<xref ref-type="table" rid="t3">Table 3</xref>).</p><table-wrap id="t3" position="float"><label>Table 3</label><caption><title>Socio-demographic characteristics of the HIV positive patients</title></caption><table frame="hsides" rules="groups"><col width="32.29%"/><col width="36.02%"/><col width="31.69%"/><thead><tr><th valign="bottom" align="left" scope="col" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)"></th><th valign="bottom" align="left" scope="col" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)"><bold>Frequency</bold></th><th valign="bottom" align="left" scope="col" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)"><bold>Percentage</bold></th></tr></thead><tbody><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Age group</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">15-24 years</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">1</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">8.3</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">25-34 years</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">2</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">16.7</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">35-44 years</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">4</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">33.3</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">45-54 years</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">3</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">25.0</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Above 55 years</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">2</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">16.7</td></tr><tr><td valign="bottom" colspan="3" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Gender</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Male</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">7</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">58.3</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Female</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">5</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">41.7</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Education</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Illiterate</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">4</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">33.3</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Less than Primary</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">4</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">33.3</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Primary completed</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">3</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">25.0</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Secondary completed</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">1</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">8.3</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Marital status</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Unmarried</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">2</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">16.7</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Currently Married</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">8</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">66.7</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Separate/Divorce</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">1</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">8.3</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Widow</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">1</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">8.3</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Occupation</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Private Service</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">1</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">8.3</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Small Business</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">1</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">8.3</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Farmer</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">1</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">8.3</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Day Labors</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">2</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">16.7</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Transport Labor</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">2</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">16.7</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Housewife</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">4</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">33.3</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Others</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">1</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">8.3</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Family type</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Nuclear Family</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">9</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">75.0</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Joint</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">3</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">25.0</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Residence</bold></td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)"></td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)"></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Peri Urb</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">2</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">16.67</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Rural</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">2</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">16.67</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Urban</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">8</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">66.66</td></tr></tbody></table></table-wrap></sec><sec><title>HIV-positive patients&apos; surgery history, contact with TB or DRTB history and their detection method</title><p>All the HIV-positive patients had co-infection with TB, and no one progressed to DR-TB. Three patients (25%) had a history of major or minor surgery, two (16.7%) had a history of close contact with TB or DRTB patient, ten (83.3%) had pulmonary involvement TB, seven bacteriologically (58.3%) detected, and nine (75.0%) were negative in both smear test and gene expert (<xref ref-type="table" rid="t4">Table 4</xref>).</p><table-wrap id="t4" position="float"><label>Table 4</label><caption><title>Distribution of the HIV-positive cases according to their surgery history and close contact with TB or DRTB history and different detection methods (n=12).</title></caption><table frame="hsides" rules="groups"><col width="56.56%"/><col width="22.01%"/><col width="21.43%"/><thead><tr><th valign="bottom" align="left" scope="col" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)"><bold>Variables</bold></th><th valign="bottom" align="left" scope="col" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)"><bold>Frequency</bold></th><th valign="bottom" align="left" scope="col" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)"><bold>Percent</bold></th></tr></thead><tbody><tr><td valign="bottom" colspan="3" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)" scope="col"><bold>Type of TB</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">TB</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">12</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">100</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">DR-TB</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">0</td><td valign="bottom" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">0</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>History of major/minor surgery</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Yes</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">3</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">25.0</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">No</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">9</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">75.0</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>History of contact with TB or DRTB patient</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Yes</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">2</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">16.7</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">No</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">7</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">58.3</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Do not Know</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">3</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">25.0</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Pulmonary involvement</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Pulmonary</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">10</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">83.3</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Extra pulmonary</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">2</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">16.7</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Detection methods</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Clinically</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">5</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">41.7</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Bacteriologically</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">7</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">58.3</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Smear test</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Smear positive</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">3</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">25.0</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Smear negative</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">9</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">75.0</td></tr><tr><td colspan="3" valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="col"><bold>Gene expert test</bold></td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Gene expert positive</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">3</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">25.0</td></tr><tr><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt" scope="row">Gene expert negative</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">9</td><td valign="top" align="left" style="border-left: solid 0.50pt; border-top: solid 0.50pt; border-right: solid 0.50pt; border-bottom: solid 0.50pt; background-color:rgb(255,255,255)">75.0</td></tr></tbody></table></table-wrap></sec></sec><sec sec-type="discussion"><title>Discussion</title><p>The prevalence of HIV among tuberculosis patients is unknown in Bangladesh. With the support of the National Tuberculosis Control Program (NTP), AIDS/STD Program (ASP), Directorate General of Health Services, Bangladesh, and the National Institute of Preventive and Social Medicine (NIPSOM), Dhaka initiated the conduct of this study financially supported by Global Fund to Fight AIDS, Tuberculosis and Malaria (GFATM). For this, a nationally representative 12,065 tuberculosis patients were selected as a sample that provides important insights into the demographic and clinical characteristics of tuberculosis (TB) patients in Bangladesh, as well as the prevalence of HIV among TB patients.</p><p>The study found that a significant proportion of TB patients were aged 55 years and older, with a mean age of 41.90 years, which is consistent with other studies that have found TB to be more prevalent among older age groups [<xref ref-type="bibr" rid="r31">31</xref>,<xref ref-type="bibr" rid="r32">32</xref>]. However, a small proportion of TB patients were under the age of 15, which may indicate the need for targeted interventions to prevent and treat TB in children. It was also observed that TB patients were more prevalent in urban areas. Numerous studies have found TB to be more prevalent in urban areas due to factors such as overcrowding and poor living conditions [<xref ref-type="bibr" rid="r33">33</xref>-<xref ref-type="bibr" rid="r36">36</xref>]. Additionally, illiteracy was the frequent educational status among TB patients, which is consistent with other studies that have found low levels of education to be a risk factor for TB [<xref ref-type="bibr" rid="r37">37</xref>-<xref ref-type="bibr" rid="r39">39</xref>].</p><p>The study also found a high prevalence of co-morbidities among TB patients, with diabetes being the most common. This is evident in other studies that have found diabetes to be a major risk factor for TB [<xref ref-type="bibr" rid="r40">40</xref>-<xref ref-type="bibr" rid="r42">42</xref>]. Additionally, the study found a low prevalence of drug-resistant TB in Bangladesh. The Government of Bangladesh has taken numerous strategies to control TB [<xref ref-type="bibr" rid="r43">43</xref>]. The lower prevalence of drug-resistant TB may reflect the success of TB control programs in Bangladesh in preventing the spread of drug-resistant strains.</p><p>The study also provides important insights into the prevalence of HIV among TB patients in Bangladesh, with a seroprevalence of 0.1%. This is lower than the global average HIV prevalence among TB patients. According to the World Health Organization (WHO), globally, approximately 8% of TB patients are co-infected with HIV [<xref ref-type="bibr" rid="r32">32</xref>]. However, this co-infection rate varies widely by region and population group. In sub-Saharan Africa, both TB and HIV co-infection are highly prevalent and can vary from 50% to 75% [<xref ref-type="bibr" rid="r44">44</xref>,<xref ref-type="bibr" rid="r45">45</xref>]. Similarly, another study found that the prevalence of HIV among TB patients can vary from 3.8 to 72.3% [<xref ref-type="bibr" rid="r9">9</xref>]. In some regions with lower rates of both diseases, the co-infection rate may be much lower. For example, numerous studies conducted in Canada found that only 1.2 to 6.5% of TB patients were also infected with HIV [<xref ref-type="bibr" rid="r46">46</xref>,<xref ref-type="bibr" rid="r47">47</xref>]. The lower prevalence of HIV among TB patients was also found to be less than 1% in Pakistan [<xref ref-type="bibr" rid="r48">48</xref>,<xref ref-type="bibr" rid="r49">49</xref>], 1.5% in Somalia [<xref ref-type="bibr" rid="r50">50</xref>], 5.54 to 9% in India [<xref ref-type="bibr" rid="r51">51</xref>-<xref ref-type="bibr" rid="r53">53</xref>], 9.9% in Nepal [<xref ref-type="bibr" rid="r12">12</xref>] and less than 1% in China [<xref ref-type="bibr" rid="r10">10</xref>].</p><p>It was found that HIV prevalence was higher among the lower educational group, with illiterate and less than primary education groups contributing the most. The majority of the HIV-positive patients were male, and most of them were currently married. Housewives accounted for the most common occupation among the positive patients. Additionally, all HIV-positive patients were also TB patients, with most of them having pulmonary involvement TB. These findings are consistent with previous studies that have shown higher HIV prevalence among individuals with lower education and socio-economic status [<xref ref-type="bibr" rid="r12">12</xref>,<xref ref-type="bibr" rid="r40">40</xref>,<xref ref-type="bibr" rid="r54">54</xref>]. It is also well-established that men are at a higher risk of HIV infection compared to women, particularly in countries with high HIV prevalence [<xref ref-type="bibr" rid="r44">44</xref>,<xref ref-type="bibr" rid="r55">55</xref>].</p><p>The results suggest that HIV prevalence was nil among DR-TB patients, indicating a high prevalence of HIV/TB co-infection and difficulty in treating this form of the disease. Regarding the medical history of the patients, a small percentage had a history of surgery, and two patients had close contact with TB or DR-TB patients, which could be contributing factors to the development of TB. Additionally, the majority of HIV-positive patients had pulmonary TB, and bacteriological detection was found in over half of the patients. These findings are consistent with previous studies that have shown a high prevalence of HIV/TB co-infection, particularly in areas with a high burden of both diseases, such as sub-Saharan Africa [<xref ref-type="bibr" rid="r55">55</xref>]. Furthermore, previous research has shown that HIV-positive individuals are at an increased risk of developing TB, with a higher incidence of pulmonary TB and extrapulmonary TB compared to HIV-negative individuals [<xref ref-type="bibr" rid="r56">56</xref>]. The lack of DR-TB among HIV-positive TB patients is a positive outcome, as DR-TB can be more difficult and costly to treat than drug-susceptible TB. However, the findings emphasize the importance of integrated HIV/TB services and the need for targeted interventions to prevent and manage HIV/TB co-infection. It is important to note that the co-infection rate among TB patients is an important public health concern, as HIV-positive individuals are at higher risk of developing active TB disease. The study recommends that all TB patients be offered HIV testing, and HIV-positive TB patients should receive antiretroviral therapy in addition to TB treatment.</p></sec><sec sec-type="conclusions"><title>Conclusion</title><p>In conclusion, the prevalence of HIV is lower than one percent. The low prevalence of HIV among TB patients in Bangladesh underscores the importance of targeted interventions to prevent the progress of HIV infection to AIDS, acquiring new TB infection and promoting both the progression of latent TB infection to active disease and relapse of the disease in previously treated patients. Special attentions are needed among patients, particularly among older age groups, urban residents, and those with co-morbidities such as diabetes.</p></sec><sec sec-type="other3"><title>Funding statement</title><p>This work was supported by the Global Fund to Fight AIDS, Tuberculosis and Malaria (GFATM) under the support of the National Tuberculosis Control Programme (NTP) and AIDS/STD Program (ASP) unit of the Directorate General of Health Services (DGHS) of the Ministry of Health and Family Welfare, Bangladesh [grant number: 338 13 09 2020]. However, the funders have no role in producing the article for journal publication.</p></sec><sec sec-type="other4"><title>Authors&apos; contribution</title><p><bold>Baizid Khoorshid Riaz</bold>- Involved in idea generation, fund acquisition, supervision and editing of the original draft to be submitted; <bold>Md. Kamrul Amin</bold>- Involved in project administration and supervision; <bold>Md. Shamiul Islam</bold>- Involved in fund acquisition and project administration; <bold>ANM Shamsul Islam, Jabin Akhter, Naznin Akter Jahan, Nasreen Farhana, Rafaat Choudhury, Mohammad Jamal Uddin, Ummul Khair Alam, Md. Nazmul Hassan Refat, Sadia Sobhan, Kamrun Nahar, Irfan Nowroze Noor, Md. Noor Ashad-Uz-Zaman and Fatima Nasreen</bold>- Involved in project administration and co-supervision; <bold>Mohammad Meshbahur Rahaman</bold>- Involved in data management, data analysis, interpretation, Writing and editing of the original draft to be submitted; <bold>Fahmida Khanam</bold>- Involved in supervision and project administration. All authors approved the original draft to be submitted.</p></sec><sec sec-type="other5"><title>Conflict interests</title><p>The authors declare that no conflict of interest exists.</p></sec><sec sec-type="other6"><title>Acknowledgment</title><p>We want to acknowledge the authority of Mycobacterial Disease Control (MBDC), Tuberculosis-Leprosy and AIDS STD Programme (TB-L &amp; ASP) Unit of the Directorate General of Health Services of the Ministry of Health and Family Welfare, Bangladesh, for their support to conduct this study.</p><sec><title>Ethical Clearance and permission of Data Collection</title><p>Ethical approval for the study was obtained from the Institutional Review Board of Bangladesh Medical Research Council (BMRC) with IRB registration number BMRC/NREC/2019-2022/993. Both verbal and written informed consent was obtained from all respondents prior to data collection. A brief introduction to the aims and objectives of the study was given first. Then, the written consent translated into the native language was read out to the illiterate tribal elderly. All collected data was kept anonymously. The design and delivery of data collection for this study continuously ensured enrolled populations&apos; rights to privacy, confidentiality, informed consent, freedom of movement, and access to information were safeguarded, and any key populations were free from discrimination, involuntary treatment, isolation, and detention.</p></sec><sec sec-type="data-availability"><title>Data availability</title><p>The data of this study is publicly available by requesting the corresponding author.</p></sec></sec></body><back><ref-list><title>References</title><ref id="r1"><label>1</label><mixed-citation publication-type="other">World Health Organization. Global tuberculosis report 2022. https://www.who.int/teams/global-tuberculosis-programme/tb-reports/global-tuberculosis-report-2022 2022.</mixed-citation></ref><ref id="r2"><label>2</label><mixed-citation publication-type="web">Centers for Disease Control and Prevention. Global Health Topics: Tuberculosis. CDC: Global Tuberculosis Fact Sheets. 2022. <ext-link ext-link-type="uri" xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="https://www.cdc.gov/globalhealth/newsroom/topics/tb/index.html">https://www.cdc.gov/globalhealth/newsroom/topics/tb/index.html</ext-link>. Accessed 11 Feb 2023.</mixed-citation></ref><ref id="r3"><label>3</label><mixed-citation publication-type="book">Tajmim T. The battle gets tougher to fight drug resistant TB. The Business Standard. 2020.</mixed-citation></ref><ref id="r4"><label>4</label><mixed-citation publication-type="web">Food and Agriculture Organization and World Bank population estimates. Population density (people per sq. km of land area) - Bangladesh. The World Bank. 2023. <ext-link ext-link-type="uri" xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="https://data.worldbank.org/indicator/EN.POP.DNST?locations=BD">https://data.worldbank.org/indicator/EN.POP.DNST?locations=BD</ext-link>. Accessed 11 Feb 2023.</mixed-citation></ref><ref id="r5"><label>5</label><mixed-citation publication-type="book">The New Age. TB kills 129 per day in Bangladesh. The New Age. 2020.</mixed-citation></ref><ref id="r6"><label>6</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Pimpin</surname> <given-names>L</given-names></string-name>, <string-name><surname>Drumright</surname> <given-names>LN</given-names></string-name>, <string-name><surname>Kruijshaar</surname> <given-names>ME</given-names></string-name>, <string-name><surname>Abubakar</surname> <given-names>I</given-names></string-name>, <string-name><surname>Rice</surname> <given-names>B</given-names></string-name>, <string-name><surname>Delpech</surname> <given-names>V</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Tuberculosis and HIV co-infection in European Union and European Economic Area countries.</article-title> <source>European Respiratory Journal.</source> <year>2011</year>;<volume>38</volume>:<fpage>1382</fpage>. <pub-id pub-id-type="doi">10.1183/09031936.00198410</pub-id><pub-id pub-id-type="pmid">21737549</pub-id></mixed-citation></ref><ref id="r7"><label>7</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Venturini</surname> <given-names>E</given-names></string-name>, <string-name><surname>Turkova</surname> <given-names>A</given-names></string-name>, <string-name><surname>Chiappini</surname> <given-names>E</given-names></string-name>, <string-name><surname>Galli</surname> <given-names>L</given-names></string-name>, <string-name><surname>de Martino</surname> <given-names>M</given-names></string-name>, <string-name><surname>Thorne</surname> <given-names>C.</given-names></string-name></person-group> <article-title>Tuberculosis and HIV co-infection in children.</article-title> <source>BMC Infect Dis.</source> <year>2014</year>;<volume>14</volume>:<fpage>S5</fpage>. <pub-id pub-id-type="doi">10.1186/1471-2334-14-S1-S5</pub-id><pub-id pub-id-type="pmid">24564453</pub-id></mixed-citation></ref><ref id="r8"><label>8</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Ali</surname> <given-names>SA</given-names></string-name>, <string-name><surname>Mavundla</surname> <given-names>TR</given-names></string-name>, <string-name><surname>Fantu</surname> <given-names>R</given-names></string-name>, <string-name><surname>Awoke</surname> <given-names>T</given-names></string-name></person-group>. <article-title>Outcomes of TB treatment in HIV co-infected TB patients in Ethiopia: a cross-sectional analytic study.</article-title> <source>BMC Infect Dis.</source> <year>2016</year>;<volume>16</volume>:<fpage>640</fpage>. <pub-id pub-id-type="doi">10.1186/s12879-016-1967-3</pub-id><pub-id pub-id-type="pmid">27814693</pub-id></mixed-citation></ref><ref id="r9"><label>9</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Belay</surname> <given-names>M</given-names></string-name>, <string-name><surname>Bjune</surname> <given-names>G</given-names></string-name>, <string-name><surname>Abebe</surname> <given-names>F.</given-names></string-name></person-group> <article-title>Prevalence of tuberculosis, HIV, and TB-HIV co-infection among pulmonary tuberculosis suspects in a predominantly pastoralist area, northeast Ethiopia.</article-title> <source>Glob Health Action.</source> <year>2015</year>;<volume>8</volume>:<fpage>27949</fpage>. <pub-id pub-id-type="doi">10.3402/gha.v8.27949</pub-id><pub-id pub-id-type="pmid">26689454</pub-id></mixed-citation></ref><ref id="r10"><label>10</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Gao</surname> <given-names>J</given-names></string-name>, <string-name><surname>Zheng</surname> <given-names>P</given-names></string-name>, <string-name><surname>Fu</surname> <given-names>H.</given-names></string-name></person-group> <article-title>Prevalence of TB/HIV Co-Infection in Countries Except China: A Systematic Review and Meta-Analysis.</article-title> <source>PLoS One.</source> <year>2013</year>;<volume>8</volume>:<elocation-id>e64915</elocation-id>-. <pub-id pub-id-type="doi">10.1371/journal.pone.0064915</pub-id><pub-id pub-id-type="pmid">23741419</pub-id></mixed-citation></ref><ref id="r11"><label>11</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Qi</surname> <given-names>C-C</given-names></string-name>, <string-name><surname>Xu</surname> <given-names>L-R</given-names></string-name>, <string-name><surname>Zhao</surname> <given-names>C-J</given-names></string-name>, <string-name><surname>Zhang</surname> <given-names>H-Y</given-names></string-name>, <string-name><surname>Li</surname> <given-names>Q-Y</given-names></string-name>, <string-name><surname>Liu</surname> <given-names>M-J</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Prevalence and risk factors of tuberculosis among people living with HIV/AIDS in China: a systematic review and meta-analysis.</article-title> <source>BMC Infect Dis.</source> <year>2023</year>;<volume>23</volume>:<fpage>584</fpage>. <pub-id pub-id-type="doi">10.1186/s12879-023-08575-4</pub-id><pub-id pub-id-type="pmid">37674103</pub-id></mixed-citation></ref><ref id="r12"><label>12</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Adhikari</surname> <given-names>N</given-names></string-name>, <string-name><surname>Bhattarai</surname> <given-names>RB</given-names></string-name>, <string-name><surname>Basnet</surname> <given-names>R</given-names></string-name>, <string-name><surname>Joshi</surname> <given-names>LR</given-names></string-name>, <string-name><surname>Tinkari</surname> <given-names>BS</given-names></string-name>, <string-name><surname>Thapa</surname> <given-names>A</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Prevalence and associated risk factors for tuberculosis among people living with HIV in Nepal.</article-title> <source>PLoS One.</source> <year>2022</year>;<volume>17</volume>:<elocation-id>e0262720</elocation-id>-. <pub-id pub-id-type="doi">10.1371/journal.pone.0262720</pub-id><pub-id pub-id-type="pmid">35089953</pub-id></mixed-citation></ref><ref id="r13"><label>13</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Bruchfeld</surname> <given-names>J</given-names></string-name>, <string-name><surname>Correia-Neves</surname> <given-names>M</given-names></string-name>, <string-name><surname>K&#x00E4;llenius</surname> <given-names>G.</given-names></string-name></person-group> <article-title>Tuberculosis and HIV Coinfection.</article-title> <source>Cold Spring Harb Perspect Med.</source> <year>2015</year>;<volume>5</volume>:<fpage>a017871</fpage>&#x2013;<lpage>a017871</lpage>. <pub-id pub-id-type="doi">10.1101/cshperspect.a017871</pub-id><pub-id pub-id-type="pmid">25722472</pub-id></mixed-citation></ref><ref id="r14"><label>14</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Hossain</surname> <given-names>MS</given-names></string-name>, <string-name><surname>Tasnim</surname> <given-names>S</given-names></string-name>, <string-name><surname>Chowdhury</surname> <given-names>MA</given-names></string-name>, <string-name><surname>Chowdhury</surname> <given-names>FIF</given-names></string-name>, <string-name><surname>Hossain</surname> <given-names>D</given-names></string-name>, <string-name><surname>Rahman</surname> <given-names>MM</given-names></string-name></person-group>. <article-title>Under-five children&apos;s acute respiratory infection dropped significantly in Bangladesh: An evidence from Bangladesh demographic and health survey, 1996&#x2013;2018. Acta Paediatrica</article-title>, <source>International Journal of Paediatrics.</source> <year>2022</year>;<volume>111</volume>:<fpage>1981</fpage>&#x2013;<lpage>1994</lpage>.<pub-id pub-id-type="pmid">35678484</pub-id></mixed-citation></ref><ref id="r15"><label>15</label><mixed-citation publication-type="other">Paul GK, Rahman MM, Naznin S, Chowdhury M, Uddin MJ. Depression and Anxiety among University Students: A Comparison between COVID-19 Pandemic Panic Period and Post-panic Period in Bangladesh. Open Access Maced J Med Sci. 2022;10 E SE-Public Health Epidemiology:52&#x2013;9.</mixed-citation></ref><ref id="r16"><label>16</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><collab>Islam MdZ</collab></person-group>, <article-title>Disu TR, Farjana S, Rahman MM. Malnutrition and other risk factors of geriatric depression: a community-based comparative cross-sectional study in older adults in rural Bangladesh.</article-title> <source>BMC Geriatr.</source> <year>2021</year>;<volume>21</volume>:<fpage>572</fpage>. <pub-id pub-id-type="doi">10.1186/s12877-021-02535-w</pub-id><pub-id pub-id-type="pmid">34663237</pub-id></mixed-citation></ref><ref id="r17"><label>17</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Paul</surname> <given-names>GK</given-names></string-name>, <string-name><surname>Rahman</surname> <given-names>MM</given-names></string-name>, <string-name><surname>Hamiduzzaman</surname> <given-names>M</given-names></string-name>, <string-name><surname>Farhana</surname> <given-names>Z</given-names></string-name>, <string-name><surname>Mondal</surname> <given-names>SK</given-names></string-name>, <string-name><surname>Akter</surname> <given-names>S</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Hypertension and its physio-psychosocial risks factors in elderly people: a cross-sectional study in north-eastern region of Bangladesh.</article-title> <source>Journal of geriatric cardiology.</source> <year>2021</year>;<volume>18</volume>:<fpage>75</fpage>&#x2013;<lpage>82</lpage>.<pub-id pub-id-type="pmid">33613661</pub-id></mixed-citation></ref><ref id="r18"><label>18</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Siddiqee</surname> <given-names>MH</given-names></string-name>, <string-name><surname>Bhattacharjee</surname> <given-names>B</given-names></string-name>, <string-name><surname>Siddiqi</surname> <given-names>UR</given-names></string-name>, <string-name><surname>Rahman</surname> <given-names>MM</given-names></string-name></person-group>. <article-title>High prevalence of vitamin D insufficiency among South Asian pregnant women: a systematic review and meta-analysis.</article-title> <source>British Journal of Nutrition.</source> <year>2021</year>;:<fpage>1</fpage>&#x2013;<lpage>12</lpage>.<pub-id pub-id-type="pmid">34725002</pub-id></mixed-citation></ref><ref id="r19"><label>19</label><mixed-citation publication-type="book">Haque MF, Rahman MM, Alif SM, Akter E, Barua S, Paul GK, et al. Estimation and prediction of doubling time for COVID-19 epidemic in Bangladesh: a modelling study of first 14 month&apos;s daily confirmed new cases and deaths. Global Biosecurity. 2021;3.</mixed-citation></ref><ref id="r20"><label>20</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Rahman</surname> <given-names>MM</given-names></string-name>, <string-name><surname>Bhattacharjee</surname> <given-names>B</given-names></string-name>, <string-name><surname>Farhana</surname> <given-names>Z</given-names></string-name>, <string-name><surname>Hamiduzzaman</surname> <given-names>M</given-names></string-name>, <string-name><surname>Chowdhury</surname> <given-names>MAB</given-names></string-name>, <string-name><surname>Hossain</surname> <given-names>MS</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Age-related Risk Factors and Severity of SARS-CoV-2 Infection: a systematic review and meta-analysis.</article-title> <source>J Prev Med Hyg.</source> <year>2021</year>;<volume>62</volume>:<fpage>E329</fpage>&#x2013;<lpage>E329</lpage>.<pub-id pub-id-type="pmid">34604574</pub-id></mixed-citation></ref><ref id="r21"><label>21</label><mixed-citation publication-type="book">UN Sustainable Development. The Sustainable Development Goals in Bangladesh. United Nations: Bangladesh. 2022.</mixed-citation></ref><ref id="r22"><label>22</label><mixed-citation publication-type="other">Geneva: World Health Organization. Roadmap towards ending TB in children and adolescents, 2nd ed. 2018.</mixed-citation></ref><ref id="r23"><label>23</label><mixed-citation publication-type="other">Mominur Rahman Md, Islam F, Saidur Rahaman Md, Sultana NA, Fahim NF, Ahmed M. Studies on the prevalence of HIV/AIDS in Bangladesh including other developing countries. Advances in Traditional Medicine. 2021. <pub-id pub-id-type="doi">10.1007/s13596-021-00610-6</pub-id>.<pub-id pub-id-type="doi">10.1007/s13596-021-00610-6</pub-id></mixed-citation></ref><ref id="r24"><label>24</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><collab>Sheikh MdT</collab></person-group>, <article-title>Uddin MdN, Khan JR. A comprehensive analysis of trends and determinants of HIV/AIDS knowledge among the Bangladeshi women based on Bangladesh Demographic and Health Surveys, 2007&#x2013;2014.</article-title> <source>Archives of Public Health.</source> <year>2017</year>;<volume>75</volume>:<fpage>59</fpage>. <pub-id pub-id-type="doi">10.1186/s13690-017-0228-2</pub-id><pub-id pub-id-type="pmid">28975026</pub-id></mixed-citation></ref><ref id="r25"><label>25</label><mixed-citation publication-type="book">National Tuberculosis Control Program, National AIDS/STD Program, World Health Organization. National Guidelines on TB/HIV Management Program Collaboration &amp; Implementation Manual: Second Edition 2016. Dhaka; 2016.</mixed-citation></ref><ref id="r26"><label>26</label><mixed-citation publication-type="book">National AIDS/STD Control, Directorate General of Health Services, Ministry of Health and Family Welfare B. National HIV Testing Services (HTS) Guideline. Dhaka; 2019.</mixed-citation></ref><ref id="r27"><label>27</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Sarwar</surname> <given-names>G</given-names></string-name>, <string-name><surname>Reza</surname> <given-names>M</given-names></string-name>, <string-name><surname>Khan</surname> <given-names>MNM</given-names></string-name>, <string-name><surname>Gourab</surname> <given-names>G</given-names></string-name>, <string-name><surname>Rahman</surname> <given-names>M</given-names></string-name>, <string-name><surname>Rana</surname> <given-names>AKMM</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Developing and testing community-based tuberculosis (TB) screening intervention to increase TB referral, case detection and knowledge among sexual minority people in urban Bangladesh: a mixed-method study protocol.</article-title> <source>BMJ Open.</source> <year>2020</year>;<volume>10</volume>:<elocation-id>e037371</elocation-id>. <pub-id pub-id-type="doi">10.1136/bmjopen-2020-037371</pub-id><pub-id pub-id-type="pmid">32963067</pub-id></mixed-citation></ref><ref id="r28"><label>28</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Porskrog</surname> <given-names>A</given-names></string-name>, <string-name><surname>Bjerregaard-Andersen</surname> <given-names>M</given-names></string-name>, <string-name><surname>Oliveira</surname> <given-names>I</given-names></string-name>, <string-name><surname>Joaqu&#x00ED;m</surname> <given-names>LC</given-names></string-name>, <string-name><surname>Camara</surname> <given-names>C</given-names></string-name>, <string-name><surname>Andersen</surname> <given-names>PL</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Enhanced tuberculosis identification through 1-month follow-up of smear-negative tuberculosis suspects.</article-title> <source>International Journal of Tuberculosis and Lung Disease.</source> <year>2011</year>;<volume>15</volume>:<fpage>459</fpage>&#x2013;<lpage>64</lpage>. <pub-id pub-id-type="doi">10.5588/ijtld.10.0353</pub-id><pub-id pub-id-type="pmid">21396203</pub-id></mixed-citation></ref><ref id="r29"><label>29</label><mixed-citation publication-type="web">World Health Organization. Data: Health data overview for the People&apos;s Republic of Bangladesh. 2022. <ext-link ext-link-type="uri" xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="https://data.who.int/countries/050">https://data.who.int/countries/050</ext-link>. Accessed 11 Jun 2024.</mixed-citation></ref><ref id="r30"><label>30</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Nazneen</surname> <given-names>A</given-names></string-name>, <string-name><surname>Tarannum</surname> <given-names>S</given-names></string-name>, <string-name><surname>Chowdhury</surname> <given-names>KIA</given-names></string-name>, <string-name><surname>Islam</surname> <given-names>MT</given-names></string-name>, <string-name><surname>Islam</surname> <given-names>SMH</given-names></string-name>, <string-name><surname>Ahmed</surname> <given-names>S</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Implementation status of national tuberculosis infection control guidelines in Bangladeshi hospitals.</article-title> <source>PLoS One.</source> <year>2021</year>;<volume>16</volume>:<elocation-id>e0246923</elocation-id>-. <pub-id pub-id-type="doi">10.1371/journal.pone.0246923</pub-id><pub-id pub-id-type="pmid">33592049</pub-id></mixed-citation></ref><ref id="r31"><label>31</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>L&#x00F6;nnroth Mario</surname> <given-names>KR</given-names></string-name></person-group>. <article-title>Global Epidemiology of Tuberculosis: Prospects for Control.</article-title> <source>Semin Respir Crit Care Med.</source> <year>2008</year>;<volume>29</volume>:<fpage>481</fpage>&#x2013;<lpage>91</lpage>. <pub-id pub-id-type="doi">10.1055/s-0028-1085700</pub-id><pub-id pub-id-type="pmid">18810682</pub-id></mixed-citation></ref><ref id="r32"><label>32</label><mixed-citation publication-type="other">World Health Organization. Global tuberculosis report 2020. 2020.</mixed-citation></ref><ref id="r33"><label>33</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Mutembo</surname> <given-names>S</given-names></string-name>, <string-name><surname>Mutanga</surname> <given-names>JN</given-names></string-name>, <string-name><surname>Musokotwane</surname> <given-names>K</given-names></string-name>, <string-name><surname>Kanene</surname> <given-names>C</given-names></string-name>, <string-name><surname>Dobbin</surname> <given-names>K</given-names></string-name>, <string-name><surname>Yao</surname> <given-names>X</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Urban-rural disparities in treatment outcomes among recurrent TB cases in Southern Province, Zambia.</article-title> <source>BMC Infect Dis.</source> <year>2019</year>;<volume>19</volume>:<fpage>1087</fpage>. <pub-id pub-id-type="doi">10.1186/s12879-019-4709-5</pub-id><pub-id pub-id-type="pmid">31888518</pub-id></mixed-citation></ref><ref id="r34"><label>34</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Sarker</surname> <given-names>M</given-names></string-name>, <string-name><surname>Homayra</surname> <given-names>F</given-names></string-name>, <string-name><surname>Rawal</surname> <given-names>LB</given-names></string-name>, <string-name><surname>Kabir</surname> <given-names>R</given-names></string-name>, <string-name><surname>Aftab</surname> <given-names>A</given-names></string-name>, <string-name><surname>Bari</surname> <given-names>R</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Urban-rural and sex differentials in tuberculosis mortality in Bangladesh: results from a population-based survey.</article-title> <source>Tropical Medicine &amp; International Health.</source> <year>2019</year>;<volume>24</volume>:<fpage>109</fpage>&#x2013;<lpage>15</lpage>. <pub-id pub-id-type="doi">10.1111/tmi.13171</pub-id><pub-id pub-id-type="pmid">30347117</pub-id></mixed-citation></ref><ref id="r35"><label>35</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Hossain</surname> <given-names>S</given-names></string-name>, <string-name><surname>Quaiyum</surname> <given-names>MA</given-names></string-name>, <string-name><surname>Zaman</surname> <given-names>K</given-names></string-name>, <string-name><surname>Banu</surname> <given-names>S</given-names></string-name>, <string-name><surname>Husain</surname> <given-names>MA</given-names></string-name>, <string-name><surname>Islam</surname> <given-names>MA</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Socio Economic Position in TB Prevalence and Access to Services: Results from a Population Prevalence Survey and a Facility-Based Survey in Bangladesh.</article-title> <source>PLoS One.</source> <year>2012</year>;<volume>7</volume>:<elocation-id>e44980</elocation-id>-. <pub-id pub-id-type="doi">10.1371/journal.pone.0044980</pub-id><pub-id pub-id-type="pmid">23028718</pub-id></mixed-citation></ref><ref id="r36"><label>36</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><collab>ABUBAKAR I</collab></person-group>, <article-title>CROFTS JP, GELB D, STORY A, ANDREWS N, WATSON JM. Investigating urban&#x2013;rural disparities in tuberculosis treatment outcome in England and Wales.</article-title> <source>Epidemiol Infect.</source> <year>2008</year>;<volume>136</volume>:<fpage>122</fpage>&#x2013;<lpage>7</lpage>. <pub-id pub-id-type="doi">10.1017/S0950268807008333</pub-id><pub-id pub-id-type="pmid">17359565</pub-id></mixed-citation></ref><ref id="r37"><label>37</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Shimeles</surname> <given-names>E</given-names></string-name>, <string-name><surname>Enquselassie</surname> <given-names>F</given-names></string-name>, <string-name><surname>Aseffa</surname> <given-names>A</given-names></string-name>, <string-name><surname>Tilahun</surname> <given-names>M</given-names></string-name>, <string-name><surname>Mekonen</surname> <given-names>A</given-names></string-name>, <string-name><surname>Wondimagegn</surname> <given-names>G</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Risk factors for tuberculosis: A case&#x2013;control study in Addis Ababa, Ethiopia.</article-title> <source>PLoS One.</source> <year>2019</year>;<volume>14</volume>:<elocation-id>e0214235</elocation-id>-. <pub-id pub-id-type="doi">10.1371/journal.pone.0214235</pub-id><pub-id pub-id-type="pmid">30939169</pub-id></mixed-citation></ref><ref id="r38"><label>38</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Narasimhan</surname> <given-names>P</given-names></string-name>, <string-name><surname>Wood</surname> <given-names>J</given-names></string-name>, <string-name><surname>MacIntyre</surname> <given-names>CR</given-names></string-name>, <string-name><surname>Mathai</surname> <given-names>D</given-names></string-name></person-group>. <article-title>Risk Factors for Tuberculosis.</article-title> <source>Pulm Med.</source> <year>2013</year>;<volume>2013</volume>:<elocation-id>828939</elocation-id>. <pub-id pub-id-type="doi">10.1155/2013/828939</pub-id><pub-id pub-id-type="pmid">23476764</pub-id></mixed-citation></ref><ref id="r39"><label>39</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Gelaw</surname> <given-names>Y</given-names></string-name>, <string-name><surname>Williams</surname> <given-names>G</given-names></string-name>, <string-name><surname>Soares Magalh&#x00E3;es</surname> <given-names>RJ</given-names></string-name>, <string-name><surname>Gilks</surname> <given-names>CF</given-names></string-name>, <string-name><surname>Assefa</surname> <given-names>Y</given-names></string-name></person-group>. <article-title>HIV Prevalence Among Tuberculosis Patients in Sub-Saharan Africa: A Systematic Review and Meta-analysis.</article-title> <source>AIDS Behav.</source> <year>2019</year>;<volume>23</volume>:<fpage>1561</fpage>&#x2013;<lpage>75</lpage>. <pub-id pub-id-type="doi">10.1007/s10461-018-02386-4</pub-id><pub-id pub-id-type="pmid">30607755</pub-id></mixed-citation></ref><ref id="r40"><label>40</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Dobler</surname> <given-names>CC</given-names></string-name>, <string-name><surname>Flack</surname> <given-names>JR</given-names></string-name>, <string-name><surname>Marks</surname> <given-names>GB</given-names></string-name></person-group>. <article-title>Risk of tuberculosis among people with diabetes mellitus: an Australian nationwide cohort study.</article-title> <source>BMJ Open.</source> <year>2012</year>;<volume>2</volume>:<elocation-id>e000666</elocation-id>. <pub-id pub-id-type="doi">10.1136/bmjopen-2011-000666</pub-id><pub-id pub-id-type="pmid">22331390</pub-id></mixed-citation></ref><ref id="r41"><label>41</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Faurholt-Jepsen</surname> <given-names>D</given-names></string-name>, <string-name><surname>Range</surname> <given-names>N</given-names></string-name></person-group>, PrayGod G, Jeremiah K, Faurholt-Jepsen M, Aabye MG, et al. <article-title>Diabetes Is a Risk Factor for Pulmonary Tuberculosis: A Case-Control Study from Mwanza, Tanzania.</article-title> <source>PLoS One.</source> <year>2011</year>;<volume>6</volume>:<elocation-id>e24215</elocation-id>-. <pub-id pub-id-type="doi">10.1371/journal.pone.0024215</pub-id><pub-id pub-id-type="pmid">21912626</pub-id></mixed-citation></ref><ref id="r42"><label>42</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Stevenson</surname> <given-names>CR</given-names></string-name>, <string-name><surname>Forouhi</surname> <given-names>NG</given-names></string-name>, <string-name><surname>Roglic</surname> <given-names>G</given-names></string-name>, <string-name><surname>Williams</surname> <given-names>BG</given-names></string-name>, <string-name><surname>Lauer</surname> <given-names>JA</given-names></string-name>, <string-name><surname>Dye</surname> <given-names>C</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Diabetes and tuberculosis: the impact of the diabetes epidemic on tuberculosis incidence.</article-title> <source>BMC Public Health.</source> <year>2007</year>;<volume>7</volume>:<fpage>234</fpage>. <pub-id pub-id-type="doi">10.1186/1471-2458-7-234</pub-id><pub-id pub-id-type="pmid">17822539</pub-id></mixed-citation></ref><ref id="r43"><label>43</label><mixed-citation publication-type="other">National Tuberculosis Control Programme (NTP). National strategic plan-public-private mix in tuberculosis, 2016-2020. 2016.</mixed-citation></ref><ref id="r44"><label>44</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><collab>World Health Organization</collab></person-group>. <source>Global tuberculosis report.</source> <year>2014</year>.</mixed-citation></ref><ref id="r45"><label>45</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Gelaw</surname> <given-names>YA</given-names></string-name>, <string-name><surname>Williams</surname> <given-names>G</given-names></string-name>, <string-name><surname>Soares Magalh&#x00E3;es</surname> <given-names>RJ</given-names></string-name>, <string-name><surname>Gilks</surname> <given-names>CF</given-names></string-name>, <string-name><surname>Assefa</surname> <given-names>Y</given-names></string-name></person-group>. <article-title>HIV Prevalence Among Tuberculosis Patients in Sub-Saharan Africa: A Systematic Review and Meta-analysis.</article-title> <source>AIDS Behav.</source> <year>2019</year>;<volume>23</volume>:<fpage>1561</fpage>&#x2013;<lpage>75</lpage>. <pub-id pub-id-type="doi">10.1007/s10461-018-02386-4</pub-id><pub-id pub-id-type="pmid">30607755</pub-id></mixed-citation></ref><ref id="r46"><label>46</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Harris</surname> <given-names>T</given-names></string-name>, <string-name><surname>Panaro</surname> <given-names>L</given-names></string-name>, <string-name><surname>Phypers</surname> <given-names>M</given-names></string-name>, <string-name><surname>Choudhri</surname> <given-names>Y</given-names></string-name>, <string-name><surname>Archibald</surname> <given-names>CP</given-names></string-name></person-group>. <article-title>HIV Testing among Canadian Tuberculosis Cases from 1997 to 1998.</article-title> <source>Canadian Journal of Infectious Diseases and Medical Microbiology.</source> <year>2006</year>;<volume>17</volume>:<elocation-id>321765</elocation-id>. <pub-id pub-id-type="doi">10.1155/2006/321765</pub-id><pub-id pub-id-type="pmid">18418494</pub-id></mixed-citation></ref><ref id="r47"><label>47</label><mixed-citation publication-type="other">Health Canada. Tuberculosis in Canada, 2020. 2022.</mixed-citation></ref><ref id="r48"><label>48</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Wali</surname> <given-names>A</given-names></string-name>, <string-name><surname>Khan</surname> <given-names>D</given-names></string-name>, <string-name><surname>Safdar</surname> <given-names>N</given-names></string-name>, <string-name><surname>Shawani</surname> <given-names>Z</given-names></string-name>, <string-name><surname>Fatima</surname> <given-names>R</given-names></string-name>, <string-name><surname>Yaqoob</surname> <given-names>A</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Prevalence of tuberculosis, HIV/AIDS, and hepatitis; in a prison of Balochistan: a cross-sectional survey.</article-title> <source>BMC Public Health.</source> <year>2019</year>;<volume>19</volume>:<fpage>1631</fpage>. <pub-id pub-id-type="doi">10.1186/s12889-019-8011-7</pub-id><pub-id pub-id-type="pmid">31801496</pub-id></mixed-citation></ref><ref id="r49"><label>49</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Hasnain</surname> <given-names>J</given-names></string-name>, <string-name><surname>Memon</surname> <given-names>GN</given-names></string-name>, <string-name><surname>Memon</surname> <given-names>A</given-names></string-name>, <string-name><surname>Channa</surname> <given-names>AA</given-names></string-name>, <string-name><surname>Creswell</surname> <given-names>J</given-names></string-name>, <string-name><surname>Shah</surname> <given-names>SA</given-names></string-name></person-group>. <article-title>Screening for HIV among tuberculosis patients: a cross-sectional study in Sindh, Pakistan.</article-title> <source>BMJ Open.</source> <year>2012</year>;<volume>2</volume>:<elocation-id>e001677</elocation-id>. <pub-id pub-id-type="doi">10.1136/bmjopen-2012-001677</pub-id><pub-id pub-id-type="pmid">23087016</pub-id></mixed-citation></ref><ref id="r50"><label>50</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Dirie</surname> <given-names>AMH</given-names></string-name>, <string-name><surname>&#x00C7;olako&#x011F;lu</surname> <given-names>S</given-names></string-name>, <string-name><surname>Abdi</surname> <given-names>BM</given-names></string-name>, <string-name><surname>Shire</surname> <given-names>AM</given-names></string-name>, <string-name><surname>Abdinur</surname> <given-names>AH</given-names></string-name></person-group>. <article-title>The prevalence of HIV among tuberculosis patients in Benadir, Somalia. Retrospective multi-center study.</article-title> <source>Annals of Medicine and Surgery.</source> <year>2022</year>;<volume>78</volume>:<elocation-id>103793</elocation-id>. <pub-id pub-id-type="doi">10.1016/j.amsu.2022.103793</pub-id><pub-id pub-id-type="pmid">35734709</pub-id></mixed-citation></ref><ref id="r51"><label>51</label><mixed-citation publication-type="other">Sawant SS, Agrawal SR, Shastri JS, Pawaskar M, Kadam P. Human Immunodeficiency Virus Infection Among Tuberculosis Patients in Mumbai. J Lab Physicians. 2011;3:012&#x2013;4.</mixed-citation></ref><ref id="r52"><label>52</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Grover</surname> <given-names>S</given-names></string-name>, <string-name><surname>Chawla</surname> <given-names>G</given-names></string-name>, <string-name><surname>Parihar</surname> <given-names>HL</given-names></string-name>, <string-name><surname>Niwas</surname> <given-names>R</given-names></string-name>, <string-name><surname>Saxena</surname> <given-names>A</given-names></string-name>, <string-name><surname>Khangarot</surname> <given-names>S</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Human immunodeficiency virus infection amongst newly diagnosed tuberculosis patients and their clinico-radiological profile: A prospective study from Western India.</article-title> <source>J Family Med Prim Care.</source> <year>2020</year>;<fpage>9</fpage>.<pub-id pub-id-type="pmid">32754523</pub-id></mixed-citation></ref><ref id="r53"><label>53</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Gothi</surname> <given-names>D</given-names></string-name>, <string-name><surname>Joshi</surname> <given-names>JM</given-names></string-name></person-group>. <article-title>Clinical and laboratory observations of tuberculosis at a Mumbai (India) clinic.</article-title> <source>Postgrad Med J.</source> <year>2004</year>;<volume>80</volume>:<fpage>97</fpage>&#x2013;<lpage>100</lpage>. <pub-id pub-id-type="doi">10.1136/pmj.2003.008185</pub-id><pub-id pub-id-type="pmid">14970298</pub-id></mixed-citation></ref><ref id="r54"><label>54</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Dahab</surname> <given-names>M</given-names></string-name>, <string-name><surname>Charalambous</surname> <given-names>S</given-names></string-name>, <string-name><surname>Hamilton</surname> <given-names>R</given-names></string-name>, <string-name><surname>Fielding</surname> <given-names>K</given-names></string-name>, <string-name><surname>Kielmann</surname> <given-names>K</given-names></string-name>, <string-name><surname>Churchyard</surname> <given-names>GJ</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>&quot;That is why I stopped the ART&quot;: Patients&apos; &amp; providers&apos; perspectives on barriers to and enablers of HIV treatment adherence in a South African workplace programme.</article-title> <source>BMC Public Health.</source> <year>2008</year>;<volume>8</volume>:<fpage>63</fpage>. <pub-id pub-id-type="doi">10.1186/1471-2458-8-63</pub-id><pub-id pub-id-type="pmid">18282286</pub-id></mixed-citation></ref><ref id="r55"><label>55</label><mixed-citation publication-type="web">UNAIDS. AIDSinfo. 2019. <ext-link ext-link-type="uri" xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="https://aidsinfo.unaids.org/">https://aidsinfo.unaids.org/</ext-link>. Accessed 19 Feb 2023.</mixed-citation></ref><ref id="r56"><label>56</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Barr</surname> <given-names>DA</given-names></string-name>, <string-name><surname>Lewis</surname> <given-names>JM</given-names></string-name>, <string-name><surname>Feasey</surname> <given-names>N</given-names></string-name>, <string-name><surname>Schutz</surname> <given-names>C</given-names></string-name>, <string-name><surname>Kerkhoff</surname> <given-names>AD</given-names></string-name>, <string-name><surname>Jacob</surname> <given-names>ST</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Mycobacterium tuberculosis bloodstream infection prevalence, diagnosis, and mortality risk in seriously ill adults with HIV: a systematic review and meta-analysis of individual patient data.</article-title> <source>Lancet Infect Dis.</source> <year>2020</year>;<volume>20</volume>:<fpage>742</fpage>&#x2013;<lpage>52</lpage>. <pub-id pub-id-type="doi">10.1016/S1473-3099(19)30695-4</pub-id><pub-id pub-id-type="pmid">32178764</pub-id></mixed-citation></ref><ref id="r57"><label>57</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Odhiambo</surname> <given-names>J</given-names></string-name>, <string-name><surname>Kizito</surname> <given-names>W</given-names></string-name>, <string-name><surname>Njoroge</surname> <given-names>A</given-names></string-name>, <string-name><surname>Wambua</surname> <given-names>N</given-names></string-name>, <string-name><surname>Nganga</surname> <given-names>L</given-names></string-name>, <string-name><surname>Mburu</surname> <given-names>M</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Provider-initiated HIV testing and counselling for TB patients and suspects in Nairobi, Kenya.</article-title> <source>Int J Tuberc Lung Dis.</source> <year>2008</year>;<volume>12</volume>:<fpage>63</fpage>&#x2013;<lpage>8</lpage>.<pub-id pub-id-type="pmid">18302825</pub-id></mixed-citation></ref><ref id="r58"><label>58</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Dimairo</surname> <given-names>M</given-names></string-name>, <string-name><surname>MacPherson</surname> <given-names>P</given-names></string-name>, <string-name><surname>Bandason</surname> <given-names>T</given-names></string-name>, <string-name><surname>Zezai</surname> <given-names>A</given-names></string-name>, <string-name><surname>Munyati</surname> <given-names>SS</given-names></string-name>, <string-name><surname>Butterworth</surname> <given-names>AE</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>The Risk and Timing of Tuberculosis Diagnosed in Smear-Negative TB Suspects: A 12 Month Cohort Study in Harare, Zimbabwe.</article-title> <source>PLoS One.</source> <year>2010</year>;<volume>5</volume>:<elocation-id>e11849</elocation-id>-. <pub-id pub-id-type="doi">10.1371/journal.pone.0011849</pub-id><pub-id pub-id-type="pmid">20676374</pub-id></mixed-citation></ref><ref id="r59"><label>59</label><mixed-citation publication-type="journal"><person-group person-group-type="author"><string-name><surname>Srikantiah</surname> <given-names>P</given-names></string-name>, <string-name><surname>Lin</surname> <given-names>R</given-names></string-name>, <string-name><surname>Walusimbi</surname> <given-names>M</given-names></string-name>, <string-name><surname>Okwera</surname> <given-names>A</given-names></string-name>, <string-name><surname>Luzze</surname> <given-names>H</given-names></string-name>, <string-name><surname>Whalen</surname> <given-names>CC</given-names></string-name>, <etal>et al.</etal></person-group> <article-title>Elevated HIV seroprevalence and risk behavior among Ugandan TB suspects: implications for HIV testing and prevention.</article-title> <source>Int J Tuberc Lung Dis.</source> <year>2007</year>.<pub-id pub-id-type="pmid">17263287</pub-id></mixed-citation></ref><ref id="r60"><label>60</label><mixed-citation publication-type="book">Wang L, Liu W, Wang L, Wang Y, Wu Z. HIV Prevalence Among Pulmonary Tuberculosis Patients in Guangxi, China. JAIDS Journal of Acquired Immune Deficiency Syndromes. 2010;53.</mixed-citation></ref></ref-list></back></article>Elizabeth KpozehouenElizabeth Kpozehouen22024-07-21T20:12:00Z2024-07-21T20:12:00Z106122699134152Microsoft Office Word032722063falsefalse154788falsefalse16.0000